Modifying Enzymes
New England Biolabs, Inc. MscI – 1250 units
A restriction endonuclease that recognizes the sequence TGG_^CCA.
New England Biolabs, Inc. EcoRI Methyltransferase – 10000 units
EcoRI Methyltransferase modifies the internal adenine residue (N6) of the sequence GAATTC.
New England Biolabs, Inc. CpG Methyltransferase (M.SssI) – 500 units
The CpG Methyltransferase, M.SssI, methylates all cytosine residues (C5) within the double-stranded dinucleotide recognition sequence 5'...CG...3'
New England Biolabs, Inc. Mung Bean Nuclease – 1500 units
Mung Bean Nuclease is a single-strand specific DNA and RNA endonuclease which will degrade single-stranded extensions leaving blunt, ligatable ends.
Takara Bio ADVANTAGE HD POLYMERASE MIX
Advantage HD DNA Polymerase Mix: this High fidelity PCR enzyme provides superb accuracy and efficiency.
New England Biolabs, Inc. Micrococcal Nuclease – 320000 gel units
Micrococcal nuclease is derived from Staphylococcus aureus and is a relatively non-specific endo-exonuclease. It is purified from a recombinant E. coli strain that digests double-stranded, single-stranded, circular and linear nucleic acids. The enzyme is active in the pH range of 7.0 - 10.0, with optimal activity at pH 9.2 for both RNA and DNA substrates. Cleavage preferences have been observed at sites rich in adenylate, deoxyadenylate or thymidylate. Both DNA and RNA are degraded to 3 phosphomononucleotides and dinucleotides. Micrococcal Nuclease works well with the SimpleChIP Enzymatic Chromatin IP Kit (Agarose Beads) from Cell Signaling Technology.
Immunology Consultants Lysosomal Acid Lipase (Host Cell Protein) ELISA Kit – CHO (Chinese Hamster Ovary)
The Lysosomal Acid Lipase (Host Cell Protein) ELISA Kit – CHO (Chinese Hamster Ovary) is a highly sensitive immunoassay designed to detect Lysosomal Acid Lipase (LAL), a lipolytic enzyme involved in the breakdown of cholesteryl esters and triglycerides within lysosomes. As a residual host cell protein released from CHO cells during biologics manufacturing, LAL may contribute to polysorbate degradation and product stability risks if not effectively removed during purification.This assay enables precise quantification of residual LAL in bioprocess samples and supports manufacturers seeking greater visibility into high-risk lipase contaminants. This kit is ideal for HCP monitoring, bioprocess development, and biopharmaceutical quality control, helping manufacturers better detect problematic lipases that may impact formulation stability and product quality.
New England Biolabs, Inc. ZraI – 200 units
A restriction endonuclease that recognizes the sequence GAC_^GTC.
Crescent Chemical Co Inc CATALASE FROM BOVINE LIVER
Catalase from bovine liver ca. 11 000 U/mg lyophil. salt-free Homogeneous in SDS-PAGE. Ca. 40 000 U/mg protein. Size - 1G Storage Conditions - -15 °C TO -25 °C Catalog Number - 26910.02 CAS 2593710
SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES ASPARTATE TRANSAMINASE AST A
NC2831157 ASPARTATE TRANSAMINASE AST A
Hampton Research Lysozyme, 8g
Crystallization grade protein standard. Please note items from this supplier are non-returnable.
Supply Solutions Liberase™ DH Research Grade, high Dispase concentration, 2x5mg
The most commonly used method for decellularization procedures, such as tissue dissociation and cell harvesting, is based on the use of proteolytic enzymes. Proteases disrupt the extracellular matrix of tissue to allow the release of individual cells or the harvesting of cultured cells for transfer. The goal of cell isolation is to maximize the yield of dissociated cells that are viable and functionally active.